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| Extraction of oils from Schizochytrium sp. and the separation and purification of high-concentration EPA/DHA |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230610 |
| KeyWord:Schizochytrium sp. algal oil extraction DHA EPA urea complexation molecular distillation isolation and purification |
| FundProject:裂殖壶菌；藻油提取；DHA；EPA；尿素包埋；分子蒸馏；分离纯化 |
| Author Name | Affiliation | | YANG Ling1,2, OU Ying1,2, QIN Yu1,2, YANG Hailin1,2 | (1. School of Biological Engineering, Jiangnan University, Wuxi 214122, Jiangsu, China
2.Key Laboratory of Industrial Biotechnology of Ministry of Education, School of
Biological Engineering, Jiangnan University, Wuxi 214122, Jiangsu, China) |
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| Abstract: |
| To realize a simple, green and efficient separation and purification process of ω-3 polyunsaturated fatty acids (ω-3 PUFAs) from algal oil, firstly, the original strain of Schizochytrium sp. and the oriented domesticated strain ALE40 were used as raw materials to extract the algal oil, the oil extraction effects of the two strains were compared through the assessment of dominance and cell permeability, and the advantages and disadvantages of three oil extraction methods （acid digestion, ultrasonic-assisted dry extraction, and ultrasonic-assisted wet extraction）were compared through the determination of the oil indexes.After ethyl esterification by transesterification of algal oil extracted by ultrasound-assisted wet extraction, high concentrations of eicosapentaenoic acid (EPA) and docosahexaenoic acid (DHA) were enriched by the combination of urea complexation and molecular distillation techniques, and the conditions of the transesterification and urea complexation processes were optimized by single factor experiment, as well as the physicochemical indexes of purified oil were determined. The results showed that compared to the original strain of Schizochytrium sp. , the cell permeability of ALE40 was significantly better, and the highest oil yield of 53.2% could be achieved by ultrasound-assisted wet extraction of ALE40 for 10 min without drying, which shortened the ultrasound time and improved the wall-breaking efficiency. In the three oil extraction methods, the algal oil extracted by ultrasound-assisted wet extraction had better physicochemical indexes.The optimal transesterification reaction conditions were reaction temperature 60 ℃, KOH addition 3%, alcohol-oil ratio 10∶ 1 and reaction time 0.5 h. The optimal urea complexation process conditions were urea-ester ratio 0.8∶ 1, solvent-ester ratio 8∶ 1, crystallization temperature 4 ℃ and crystallization time 8 h. After urea complexation, the algal oil was purified by secondary molecular distillation, the total content of EPA and DHA in the algal oil increased from 1740% (transesterification reaction) to 90.86%, and the total recovery was 62.28%. The physicochemical indexes of the purified algal oil met the requirements of the industry standard, and there was no precipitation within three months of storage, with a certain degree of stability. In conclusion, the extraction, separation and purification process can realize the recyling of reagents throughout the entire procedure, and the prepared algal oil has low acid value and no peculiar odor. |
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