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| In vitro antioxidant activity of polysaccharide from perilla seed meal and its protective effects against oxidative stress-induced damage of hepatocytes |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230284 |
| KeyWord:perilla polysaccharide free radical scavenging hepatocytes oxidative damage cell apoptosis |
| FundProject:国家自然科学基金（81603150）；江苏师范大学研究生科研与实践创新计划（2022XKT0895） |
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| Abstract: |
| To provide reference for the high-value utilization of perilla seed meal, perilla polysaccharide (PMP) prepared from perilla seed meal by water solution and alcohol precipitation was used as material, and its in vitro antioxidant activity was analyzed by detecting the scavenging rate of hydroxyl free radical (·OH), superoxide anion radical(O-2·), 1,1-diphenyl-2- picrylhydrazyl radical (DPPH·), nitrite (NO-2). At the same time, H2O2 was used to establish LO2 hepatocyte damage model, and the antioxidant ability of PMP was investigated in cellular level. The protective mechanism of PMP on LO2 hepatocytes oxidative stress damage was further studied. The results showed that PMP had certain scavenging ability to ·OH, O-2·, DPPH· and NO-2, and their EC50 values were 964.59，6 376.84，333.55，275.24 μg/mL, respectively. A concentration of 800 μmol/L H2O2 treatment for 24 h was selected as the construction condition for the oxidative damage model of LO2 cells. PMP could significantly increase the viability of LO2 cells with H2O2-induced damage, and the cell viability increased to 92.46% when the PMP mass concentration was 1 000 μg/mL. In terms of the mechanism of protecting LO2 cells from oxidative damage, PMP significantly reduced the level of intracellular reactive oxygen species (ROS) induced by H2O2, increased mitochondrial membrane potential, increased the content of glutathione (GSH) and the activities of superoxide dismutase (SOD) and catalase (CAT) in cells, reduced the level of malondialdehyde (MDA), and improved the apoptosis caused by oxidative stress damage by significantly up-regulating the expression of apoptotic protein Bcl-2 and down-regulating the expression of Bax. In summary, PMP exhibit certain free radical scavenging activity and protective effects on LO2 cells damaged by H2O2. |
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