川渝火锅用牛油关键风味物质的鉴定
Identification of key flavour substances of beef tallow used in Sichuan-Chongqing hotpot
  
DOI:10.19902/j.cnki.zgyz.1003-7969.230103
中文关键词:  川渝火锅  牛油风味  分子感官科学  关键风味物质
英文关键词:Sichuan-Chongqing hotpot  beef tallow flavour  molecular sensory science  key flavour substances
基金项目:国家重点研发计划资助(2021YFD2100303)
Author NameAffiliation
YOU Yue1, WANG Qiaojun2, QIN Yali1, YANG Lixue2, ZHANG Yiren1, ZHANG Hui1, WU Gangcheng1, WANG Xingguo1 (1.School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China
2.Guanghan Maidele Food Co. , Ltd. , Deyang 618000, Sichuan, China) 
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中文摘要:
      旨在为不同风味需求火锅底料的研究提供参考依据,通过消费者偏好分析和挥发性风味成分的含量与种类从6种牛油(3种市售牛油、未精炼牛油、脱胶牛油、精炼牛油)中筛选出最受消费者喜爱的牛油,并采用顶空固相微萃取结合气相色谱-质谱联用技术、香味提取物稀释分析法、气味活度值法、风味重组和风味缺失实验对其风味物质进行鉴定。结果表明:精炼牛油的消费者偏好平均得分最高且挥发性物质组成最丰富,其含有23种风味物质,包括醛类10种、酸类5种、杂环类4种、醇类3种和酮类1种;根据牛油风味物质的气味活度值,确定牛油的7种关键风味物质为(E,E)-2,4-壬二烯醛、(E)-2-辛烯醛、乙酸、2-戊基吡啶、辛醛、1-辛烯-3-醇和2-戊基呋喃,通过风味重组和风味缺失实验得出(E,E)-2,4-壬二烯醛、(E)-2-辛烯醛、乙酸和辛醛对牛油风味的贡献最大,2-戊基吡啶、1-辛烯-3-醇和2-戊基呋喃对牛油风味的贡献次之。综上,确认了牛油中7种关键风味物质,可为牛油产品风味的合理开发提供参考。
英文摘要:
      In order to establish a foundation for researching the diverse flavour requirements of substances in hotpot bases, six types of beef tallow(three types of commercially available beef tallow, unrefined beef tallow, degummed beef tallow, and refined beef tallow) were used as materials, the most popular beef tallow was selected based on customer preference analysis and the content and type of volatile flavour components, and the flavour substances of the most popular beef tallow were identified using headspace solid-phase microextraction-gas chromatography - mass spectrometry, dilution analysis of flavour extracts, odour activity values, flavour reconstitution and flavour deletion experiments. The results indicated that refined beef tallow received the highest average score in the consumer preference analysis and had the most substantial chemical composition with 23 volatile substances including 10 kinds aldehydes, 5 kinds acids, 4 kinds heterocyclic, 3 kinds alcohols and 1 kind ketone compounds. Based on the odour activity values of beef tallow flavour substances, 7 kinds key flavour substances of beef tallow were identified as (E,E)-2,4-nonadienal, (E)-2-octenal, acetic acid, 2-pentylpyridine, octanal, 1-octen-3-ol and 2-pentylfuran. Flavour reconstitution and deletion experiments showed that (E,E)-2,4-nonadienal, (E)-2-octenal, acetic acid and octanal contributed the most to beef tallow flavour, followed by 2-pentylpyridine, 1-octen-3-ol and 2-pentylfuran. In conclusion, the 7 kinds key flavour substances are determined in the beef tallow, which can provide a reference for the rational development of beef tallow product flavours.
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