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| Extraction and structural characteristics of phospholipid DHA from grassland Sunite sheep brain |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240215 |
| KeyWord:grassland Sunite sheep brain phospholipid DHA sn-2 position fatty acid fatty acid composition DHA content |
| FundProject:内蒙古自治区自然科学基金项目（2023MS03034）；内蒙古自治区科技计划项目（2023YFDZ0050）；内蒙古自治区科技计划项目（2021GG0348）；草原英才项目（内组通字〔2021〕333号，内人才发〔2023〕3号） |
| Author Name | Affiliation | | BAOYINDUGURONG ·Jinhua1,2,3，HESHUOTE Mailisi4，
HUGEJILETU3，HOU Ronglun3，YU Bo1,2，ZHOU Huanmin2 | （1.College of Food Science and Engineering, Inner Mongolia Agricultural University, Hohhot 010010, China
2.Inner Mongolia Key Laboratory of Biofabrication, Hohhot 010010, China
3.Inner Mongolia Hesige
Agriculture and Animal Husbandry Technology Co. , Ltd. , Xilingol League 026321, Inner
Mongolia, China
4.Crystal Pharmatech, Inc. , Cranbury, NJ 08512, USA） |
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| Abstract: |
| To provide scientific data for the development and utilization of phospholipid docosahexaenoic acid (DHA-PL) in green natural grassland sheep brain, the crude fat was extracted with solvent from the freeze-dried Sunite sheep brain and precipitated with ice acetone to obtain phospholipid, and then the phosphatidylcholine (PC) and phosphatidylserine (PS) were separated by silica gel column chromatography from phospholipid. The total cholesterol content in the crude fat was determined, as well as the PC and PS contents in the phospholipid. The contents of DHA in crude fat, phospholipid, PC and PS, and the contents of DHA at sn-2 position in PC and PS were analyzed. The results showed that the higher crude fat yield of 45% was obtained by extraction with ethanol-n-hexane (volume ratio of 2∶ 1). The cholesterol content in the crude fat was 29.42 mg/g, and the DHA content in the crude fat was 8.89%. The DHA content in the phospholipid was 1141%. The contents of PC and PS in phospholipid were 27.67 μg/g and 10.69 μg/g, respectively. The DHA content in the sn-2 position of PC and PS was 8.06% and 2.59%, respectively. In summary, DHA-PL that is easily absorbed can be prepared from the Sunite sheep brain. |
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