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| Production of fatty acids from acidified oil catalyzed by magnetic chitosan immobilized Candida rugosa lipase |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230062 |
| KeyWord:immobilization lipase hydrolysis acidified oil fatty acid |
| FundProject:国家自然科学基金资助项目（21978112） |
| Author Name | Affiliation | | ZHANG Pingbo1, TENG Shiqi1, WANG Pengfei2, FAN Xiulin1,
YAN Qin2, FAN Mingming1 | (1.Key Laboratory of Synthetic and Biological Colloids of Minstry of Education, School of
Chemical and Material Engineering, Jiangnan University, Wuxi 214122, Jiangsu, China
2.Nantong Jinli Oil Industry Co. , Ltd. , Nantong 226017, Jiangsu, China) |
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| Abstract: |
| To explore and establish strategies for the application of biocatalysis in the utilization of oil by-products, Candida rugosa lipase (CRL) was immobilized on magnetic chitosan (Fe3O4@CS) to prepare magnetic immobilized lipase (Fe3O4@CS-CRL). The Fe3O4 @ CS-CRL was characterized by X-ray diffraction (XRD), scanning electron microscopy (SEM) and vibrating sample magnetometer (VSM). The lipase loading, specific activity and enzymatic stability of the Fe3O4@CS-CRL were determined. The Fe3O4@CS-CRL was used to catalyze the hydrolysis of acidified soybean oil to produce fatty acids, and the effect of the catalytic reaction conditions on the hydrolysis rate was studied through single factor experiment, and the reuse performance of the catalyst was investigated.The results showed that Fe3O4@CS-CRL retained the magnetic crystal form of Fe3O4, with spherical granule, saturation magnetization strength of 61.1 emu/g, lipase load of 5.53 mg/g, and specific activity of 7.70 U/g. Compared with free CRL, Fe3O4@CS-CRL had better thermal, pH, organic solvent and storage stability, and the optimal reaction conditions for the production of fatty acids from acidified soybean oil catalyzed by Fe3O4@CS-CRL were reaction temperature 40 ℃, water-oil volume ratio 2∶ 1(PBS to acidified soybean oil), enzyme dosage 15% (based on the mass of acidified soybean oil), and reaction time 36 h. Under the optimal conditions, the hydrolysis rate of acidified soybean oil reached 84.93%. In addition, the catalyst Fe3O4@CS-CRL maintained 61.4% of its initial activity after five times of reuse. In conclusion, the Fe3O4@CS-CRL catalytic reaction in acidified oil broadens the application range of immobilized CRL. The catalytic reaction system is a sustainable and efficient use of by-products of oil refining industry, and has a good prospect in replacing the traditional industrial reaction with high energy consumption. |
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