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| Synthesis of laurate-diglyceride and myristate-diglyceride catalyzed by immobilized lipase R IM-03 and their purification |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230589 |
| KeyWord:diacylglycerol immobilized lipase R IM-03 enzymatic synthesis DAG/(DAG+TAG) melting property |
| FundProject: |
| Author Name | Affiliation | | FANG Mingxuan1, CHEN Yang2, JIN Jun1,3, JIN Qingzhe1, WANG Xingguo1,3 | (1.School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China
2.Wuxi Vland Biotech Co. , Ltd. , Wuxi 214122, Jiangsu, China
3.Food Laboratory of
Zhongyuan, Luohe 462300, Henan, China) |
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| Abstract: |
| In order to promote application of immobilized lipase R IM-03 in synthesis of diacylglycerols(DAG), laurate-diglyceride and myristate-diglyceride were synthesized by immobilized lipase R IM-03 catalysis, and purified with molecular distillation. The processing conditions of esterification and molecular distillation were optimized, and the physicochemical properties of the purified diacylglycerols were evaluated. The results showed that the optimal conditions for the synthesis of diacylglycerols from glycerol and lauric acid or myristic acid catalyzed by immobilized lipase R IM-03 were as follows: molar ratio of substrate (fatty acid to glycerol)2∶ 1, reaction temperature 60 ℃, reaction time 6 h, and enzyme addition amount 6%(based on the mass of substrate). Under these conditions, the content of laurate-diglyceride and myristate-diglyceride in the crude products were 43.87% and 30.00%, respectively, and the contents of triacylglycerol (TAG) were 8.71% and 6.87%, respectively. The catalyst could be reused at least 10 times. The optimal conditions for purification of laurate-diglyceride were as follows: the primary molecular distillation temperature 170 ℃, and the secondary molecular distillation temperature 180 ℃. The optimal conditions for purification of myristate-diglyceride were as follows: the primary molecular distillation temperature 170 ℃, and the secondary molecular distillation temperature 190 ℃. Under these conditions, the contents of diacylglycerols in purified laurate-diglyceride and myristate-diglyceride reached 81.29% and 80.08%, respectively. The contents of 1,3- diacylglycerols were 53.16% and 54.27% respectively, and the DAG/(DAG+TAG) was about 80%. Slip melting points of the purified laurate-diglyceride and myristate-diglyceride were 48.9 ℃ and 57.0 ℃, respectively, and the solid fat content showed a steep decline at 30-40 ℃. In the mixing system of the two diacylglycerols, when the myristate-diglyceride content was more than 60%, the compatibility was desirable. In conclusion, immobilized lipase R IM-03 exhibits potential application in synthesis of diacylglycerol. DAG/(DAG+TAG) can be used as a key control index, and the melting property of the product can be changed by combination of different fatty acid-diglyceride. |
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