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| Optimization of ultrasonic-assisted extraction process and antioxidant activity of polysaccharides from oil-tea camellia seed hull |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230631 |
| KeyWord:oil-tea camellia seed hull polysaccharides response surface methodology ultrasonic-assisted extraction antioxidant activity |
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| Abstract: |
| In order to provide a basis for the development and utilization of oil-tea camellia seed hull polysaccharides, using oil-tea camellia seed hull as raw material, the process conditions of ultrasonic-assisted extraction of oil-tea camellia seed hull polysaccharides (CFP-U)were optimized through single factor test and response surface methodology. The physical and chemical properties (molecular weight, monosaccharide composition, Fourier transform infrared spectroscopy, ultraviolet spectroscopy, microstructure) and in vitro antioxidant activity (scavenging rates of hydroxyl radical, superoxide anion radical and ABTS+ radical) of CFP-U were determined. The results showed that the optimal ultrasonic-assisted extraction conditions of CFP-U were ultrasonic time 20 min, ultrasonic power 650 W and liquid-solid ratio 40∶ 1, and the yield of CFP-U reached (3.28±0.06)% under these conditions. High performance gel permeation chromatography analysis showed that the purity of CFP-U was high, and its molecular weight was 91.6 kDa. High performance liquid chromatography analysis showed that the CFP-U was composed of mannose, rhamnose, glucuronic acid, galacturonic acid, glucose, galactose, xylose and fucose in an average molar ratio of 005∶ 0.14∶ 005∶ 1.00∶ 005∶ 0.30∶ 0.35∶ 0.41. The infrared spectroscopy showed that there were α-glucoside bond, β-glucoside bond and pyranose ring in CFP-U. Full-wavelength ultraviolet scanning showed that CFP-U contained almost no protein. CFP-U was observed by scanning electron microscope with irregular shape, smooth surface and sheet structure. The determination of antioxidant activity in vitro showed that CFP-U could effectively scavenge hydroxyl radical, superoxide anion radical and ABTS+ radical in a dose-response relationship within a certain concentration range, and the antioxidant activity of CFP-U was higher than the polysaccharides extracted by traditional hot water method. In conclusion, the ultrasonic-assisted extraction method can effectively obtain polysaccharides from oil-tea camellia seed hull, and the polysaccharides extracted has high antioxidant capacity in vitro. |
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