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| Preparation of biodiesel from acidified rice bran oil catalyzed by Lipozyme TL100L |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230628 |
| KeyWord:acidified rice bran oil lipase esterification biodiesel |
| FundProject:河南省科技厅自然科学项目(232103810067)；河南工业大学自然创新基金支持计划(2020ZKCJ10) |
| Author Name | Affiliation | | DOU Weiguo1,2, CHEN Mingxia1, MENG Xiaopeng3,
WU Anshi4, BI Yanlan1,2, CHEN Jingnan1,2 | (1.College of Food Science and Technology, Henan University of Technology, Zhengzhou 450001, China
2.Food Laboratory of Zhongyuan, Luohe 462300, Henan, China
3.Zhengzhou Ocean Oil Engineering
Co. , Ltd. , Zhengzhou 450018, China
4.Zhejiang Delekang Food Co. , Ltd. , Taizhou 317306,
Zhejiang, China) |
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| Abstract: |
| Aiming to provide reference for the industrial application of acidified rice bran oil, biodiesel was prepared by enzyme catalysis of acetone extract of acidified rice bran oil. The effects of lipase type, enzyme dosage, alcohol-oil molar ratio and reaction temperature on biodiesel yield were investigated through single factor experiments. The process conditions for biodiesel preparation were optimized by orthogonal experiments. The biodiesel prepared was refined by molecular distillation, and the refined biodiesel was characterized. The results showed that after the pretreatment of the acidified rice bran oil with acetone extraction, the peroxide value, oryzanol content and unsaponifiable matters content did not change significantly, the acid value and saponification value increased significantly, and the contents of moisture and volatile matter, phospholipid, ash and soap reduced significantly. The optimal process conditions for biodiesel preparation were as follows: alcohol-oil molar ratio 3∶ 1, liquid lipase Lipozyme TL100L dosage 4%, reaction temperature 30 ℃, and reaction time 4 h. Under the optimal conditions, the biodiesel yield reached (86.77±0.54)%, and the content of fatty acid methyl ester reached (97.00±0.04)% after purification by molecular distillation. The physicochemical indexes of refined biodiesel were in accordance with the requirements of GB 25199-2017. Fourier infrared spectroscopy (FTIR) analysis confirmed that the product contained fatty acid methyl ester, thermogravimetric (TG-DTG) analysis showed that the weight loss of refined biodiesel was the fastest in the temperature interval of 178.0-293.2 ℃; differential scanning calorimetry (DSC) analysis showed that the peaks at temperatures from -37.5 ℃ to -47.2 ℃ were related to the crystals of unsaturated fatty acid methyl ester, and the peaks at temperatures from -7.9 ℃ to -3.7 ℃ were related to the crystals of saturated fatty acid methyl ester, and the crystallisation starting temperature of biodiesel was -8.87 ℃. In conclusion, the enzymatic preparation of biodiesel from acidified rice bran oil has great potential for development. |
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