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| Determination of three vanillin compounds in edible oil by UPLC |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230138 |
| KeyWord:UPLC vegetable oil vanillin methyl vanillin ethyl vanillin |
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| Abstract: |
| In order to solve the problem of incomplete purification in the pretreatment of the existing testing methods,a method for the determination of vanillin, methyl vanillin, and ethyl vanillin in edible oil by ultra high performance liquid chromatography (UPLC) was established. The target compounds in edible oil sample were extracted with acetonitrile, purified by n-hexane saturated by acetonitrile, frozen and stratified, then extracted and purified by Oasis PRiME HLB column, separated by C18 chromatographic column, and eluted with 0.5% formic acid aqueous solution and acetonitrile as the mobile phase gradient for the UPLC analysis. The external standard method was used for quantification. The results showed that the linearity of the three vanillins was good in the range of 0.1-100.0 mg/L, with correlation coefficient higher than 0.999, the detection limit was 0.03 mg/kg, and the quantitation limit was 0.1 mg/kg.The average recoveries at three additive concentrations of 0.1, 0.2 mg/kg and 1.0 mg/kgwere 86.6%- 108.1%, and the RSD was 2.0%-8.8%. There was no significant difference between the results of the established method and BJS 201705.The established method uses a two-step purification to reduce the interference of impurities to vanillin compounds, and is suitable for the determination of vanillins in edible oil. |
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