Effect of Aspergillus oryzae fermentation on the feeding quality of cottonseed shells
  
DOI:10.19902/j.cnki.zgyz.1003-7969.230681
KeyWord:Aspergillus oryzae  solid state fermentation  cottonseed shell  gossypol  feeding quality
FundProject:第二师铁门关市科技攻关(2023GG2504);兴安盟科技计划项目
Author NameAffiliation
CHEN Tianming1, LI Xinmin2, ZHANG Guoyu2, ZHAO Haishan3, LU Jian1, CAI Guolin1 (1.National Engineering Research Center for Cereal Fermentation and Food Biomanufacturing, Jiangnan UniversityWuxi 214122, Jiangsu, China 2.Xinjiang Tianmu Biotechnology Co. , Ltd. , Tiemenguan 841000, Xinjiang, China 3.Jiahe Animal Husbandry Co. , Ltd. , in Ulanhot, Ulanhot 137400, Inner Mongolia, China) 
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Abstract:
      In order to improve the feeding quality of cottonseed shells, the effects of Aspergillus oryzae TM-1 fermentation on the degradation of gossypol and fiber-like substance, the improvement of protein quality and the accumulation of beneficial metabolites in cottonseed shells were studied. The results showed that after fermentation, the gossypol content in cottonseed shells decreased from 645.3 mg/kg to 137.2 mg/kg, and the degradation rate reached 78.7%. The structure of cottonseed shells fermented was loose and the gap was large, and the contents of lignin, cellulose, hemicellulose, neutral detergent fiber and acid detergent fiber decreased significantly, indicating that the structural barrier affecting the digestion and utilization of cottonseed shells was effectively degraded. The contents of total acid and eight organic acids in cottonseed shells increased significantly, and the content of malic acid increased by 492 times, reaching 734.0 mg/100 g. The content of crude protein in cottonseed shells increased from 12.5% to 15.5%, the proportion of acid-soluble protein increased from 4.8% to 13.5%, the content of oligopeptides with molecular weight below 1 000 Da accounted for 83.0%, and the total free amino acids increased by 115.7%. The limiting amino acids lysine and histidine increased by 117.4% and 64.2%, respectively. The fermented cottonseed shells were rich in hydrolytic enzymes, including neutral protease, cellulase, β-glucanase and β-1,4 endoxylanase, and their enzyme activities were 245.2, 13.4, 39.7 U/g and 19.7 U/g, respectively. In summary, the feeding quality of cottonseed shells is significantly improved by Aspergillus oryzae TM-1 fermentation.
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