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| Characterization of diacylglycerol isomers in flaxseed diacylglycerol oil using GC-MS |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230643 |
| KeyWord:SPE diacylglycerol isomers GC-MS EIC |
| FundProject:广东省基础与应用基础研究基金（2021A1515012504） |
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| Abstract: |
| Aiming to provide technical reference for the detection and analysis of diacylglycerol in vegetable oils, the diacylglycerol in flaxseed diacylglycerol oil was analysed by gas chromatography-mass spectrometry (GC-MS) after purification by two stage solid phase extraction using flaxseed diacylglycerol oil as the test sample, the composition of the diacylglycerol was analysed based on the characteristic ionic fragments of the diacylglycerol, the composition of the diacylglycerol was analysed through the total ion current chromatogram (TIC) and the extracted ion chromatogram (EIC) for the study of isomers and compared with the high performance liquid chromatography (HPLC) method for the determination of diacylglycerol. The results showed that high purity diacylglycerol could be obtained after two stage solid phase extraction of the flaxseed diacylglycerol oil, and the separation effect of the Rxi-65TG GC column was better than that of the DB-5ms. The results of the characteristic ionic fragmentation analysis and the EIC indicated that the flaxseed diacylglycerol oil contained a total of 20 kinds of diacylglycerols, which mainly consisted of 1-linoleic-3-linolenic acid diacylglycerol(21.36%), 1, 3-di-linolenic acid diacylglycerol(16.57%) and 1-oleic acid-3-linolenic acid diacylglycerol (15.75%). Compared with HPLC, GC-MS could achieve accurate characterization and quantification of diacylglycerol, and the peak separation was better than that of HPLC. In conclusion, GC-MS can be used for the analysis of the composition of diacylglycerol in vegetable oils. |
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