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| QuEChERS pre-processing technology for detecting four synthetic antioxidants in edible vegetable oil |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230669 |
| KeyWord:QuEChERS high performance liquid chromatography synthetic antioxidant edible vegetable oil |
| FundProject:上海市“科技创新行动计划”科学仪器和化学试剂项目(21142203200) |
| Author Name | Affiliation | | ZHU Rui1,2，XUE Bin1,2，REN Xingfa2,3 | （1.Shanghai Weilexi Testing Technology Co. , Ltd. ，Shanghai 201613，China； 2.Welch Materials(Shanghai),
Inc. , Shanghai 201613, China； 3.Shanghai Engineering Research Center of Food Rapid Detection,
University of Shanghai for Science and Technology, Shanghai 200093, China） |
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| Abstract: |
| In order to improve the efficiency, convenience, and safety of the detection of synthetic antioxidants in edible vegetable oil, QuEChERS pre-processing technology was used to establish a sample pretreatment method for the rapid and efficient extraction, separation, and purification of four synthetic antioxidants from edible vegetable oil, namely propyl gallate (PG), tert-butyl hydroquinone (TBHQ), butyl hydroxyanisole (BHA), and butylated hydroxytoluene (BHT) for detection by high performance liquid chromatography. The sample was first dissolved in acetonitrile-ethyl acetate solution (volume ratio 3∶ 7) and added to a specialized extraction adsorbent for synthetic antioxidants. Then, the extraction solution(adding 0.02% α-lipoic acid as a protective agent) was added for 1 min of oscillation extraction. After centrifugation, the upper layer of extraction solution was added to the purification adsorbent of C18E for 2 min of oscillation. After centrifugation, the upper layer of clear liquid was taken and the content of four antioxidants was measured using a high performance liquid chromatography equipped with diode array detector. At the same time, the oil residue rate in the final extraction solution for chromatographic determination was measured. The results showed that the four antioxidants had a good linear relationship within the range of 1.0-100.0 mg/L, and the detection limits was 4.0 mg/kg. The spiked recovery rate at four different levels of 10, 30, 200 mg/kg and 300 mg/kg was 82.08%-102.99%, and the relative standard deviation (RSD) was 080%-4.97%. The average oil residue rate in the final extract solution of antioxidants was 0.006 1 g/mL, with a RSD of 481%. In conclusion, this QuEChERS pre-processing technology established has the characteristics of simple and fast operation, high pretreatment efficiency, good accuracy of results, and good purification effect, and is suitable for the detection and analysis of four synthetic antioxidants in edible vegetable oils. |
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