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| 瓜蒌籽甘油二酯油的酶法甘油解制备研究 |
| reparation of diacylglycerol from Trichosanthes kirilowii seed oil by enzymatic glycerolysis |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230395 |
| 中文关键词: 瓜蒌籽油 甘油二酯 酶法甘油解法 |
| 英文关键词:Trichosanthes kirilowii seed oil diglycerides enzymatic glycerolysis |
| 基金项目:安徽省农业农村厅产业竞争力提升科技行动项目(AHCYJSTX-07-202003)；安徽农业大学神农新秀项目（rc352102）；安徽农业大学大学生创新项目（X202210364098） |
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| 中文摘要: |
| 为了优化瓜蒌籽甘油二酯（DAG）油制备工艺，提高DAG工业化产量，以脂肪酶为催化剂，采用酶法甘油解法制备瓜蒌籽DAG油，通过单因素试验探究了反应时间、脂肪酶添加量、醇油质量比对DAG含量的影响，通过响应面法优化反应条件，并对酶法甘油解反应前后瓜蒌籽油的脂质组成、脂肪酸组成和基本理化指标进行分析。结果表明：瓜蒌籽DAG油最佳制备条件为反应时间12.5 h、醇油质量比1∶ 10、脂肪酶添加量6.5%（以醇油总质量计），该条件下所得瓜蒌籽DAG油的DAG含量为60.60%，其中1,3-DAG含量为45.97%，1,2-DAG含量为14.63%；与酶法甘油解反应前比较，瓜蒌籽DAG油的主要脂肪酸组成并没有改变，但酸值上升，碘值和皂化值下降。综上，采用酶法甘油解法可生产出DAG含量较高的瓜蒌籽DAG油。 |
| 英文摘要: |
| In order to optimize the preparation process of Trichosanthes kirilowii seed diglycerides (DAG) oil and improve the industrial yield of DAG, enzymatic glycerolysis method was used to prepare Trichosanthes kirilowii seed DAG oil with lipase as catalyst. The effects of reaction time, enzyme addition amount and glycerol to oil mass ratio on the content of DAG were investigated through single factor test. The reaction conditions were optimized by response surface methodology. The lipid composition, fatty acid composition and basic physicochemical indexes of Trichosanthes kirilowii oil before and after enzymatic glycerolysis were analyzed. The results showed that the optimal preparation conditions were reaction time 12.5 h, glycerol to oil mass ratio 1∶ 10 and enzyme addition amount 6.5% (base on the total mass of glycerol and oil). The DAG content of Trichosanthes kirilowii seed DAG oil was 60.60% under these conditions, in which 1, 3-DAG content was 45.97% and 1,2-DAG content was 14.63%. Compared with Trichosanthes kirilowii seed oil, the main fatty acid composition of Trichosanthes kirilowii seed DAG oil did not change, but the acid value increased, while the iodine value and saponification value decreased. In conclusion, Trichosanthes kirilowii seed DAG oil with high DAG content can be produced by enzymatic glycerolysis method. |
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