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| reparation of diacylglycerol from Trichosanthes kirilowii seed oil by enzymatic glycerolysis |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230395 |
| KeyWord:Trichosanthes kirilowii seed oil diglycerides enzymatic glycerolysis |
| FundProject:安徽省农业农村厅产业竞争力提升科技行动项目(AHCYJSTX-07-202003)；安徽农业大学神农新秀项目（rc352102）；安徽农业大学大学生创新项目（X202210364098） |
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| Abstract: |
| In order to optimize the preparation process of Trichosanthes kirilowii seed diglycerides (DAG) oil and improve the industrial yield of DAG, enzymatic glycerolysis method was used to prepare Trichosanthes kirilowii seed DAG oil with lipase as catalyst. The effects of reaction time, enzyme addition amount and glycerol to oil mass ratio on the content of DAG were investigated through single factor test. The reaction conditions were optimized by response surface methodology. The lipid composition, fatty acid composition and basic physicochemical indexes of Trichosanthes kirilowii oil before and after enzymatic glycerolysis were analyzed. The results showed that the optimal preparation conditions were reaction time 12.5 h, glycerol to oil mass ratio 1∶ 10 and enzyme addition amount 6.5% (base on the total mass of glycerol and oil). The DAG content of Trichosanthes kirilowii seed DAG oil was 60.60% under these conditions, in which 1, 3-DAG content was 45.97% and 1,2-DAG content was 14.63%. Compared with Trichosanthes kirilowii seed oil, the main fatty acid composition of Trichosanthes kirilowii seed DAG oil did not change, but the acid value increased, while the iodine value and saponification value decreased. In conclusion, Trichosanthes kirilowii seed DAG oil with high DAG content can be produced by enzymatic glycerolysis method. |
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