| In order to enhance the glycerolysis reaction of soybean oil catalyzed by enzyme, and provide technical support for the industrial production of diacylglycerol oil, diacylglycerol oil was prepared by microchannel reactor using soybean oil and glycerol as raw materials and liquid lipase as catalyst. The effects of dosage of liquid lipase, mass ratio of soybean oil to glycerol, reaction temperature and reaction time on the mass fraction of diacylglycerol in the crude product were investigated by single factor experiment. The stability of the process was studied by amplification experiment, and the amplification crude product was purified by molecular distillation. The results showed that the optimal conditions for the preparation of diacylglycerol oil were obtained as follows: dosage of liquid lipase (10 000 U/mL) 3% (on the basis of soybean oil mass), mass ratio of soybean oil to glycerol 10∶ 1, reaction temperature 50 ℃ and reaction time 9.0 s. Under these conditions, the mass fraction of diacylglycerol in the crude product reached 44%. After amplification experiments, there was no significant change in the mass fraction of components (free fatty acid, monoacylglycerol, diacylglycerol, triacylglycerol) in the crude product, with the mass fraction of diacylglycerol 42%, and the reaction could proceed continuously in the microchannel reactor. After molecular distillation purification, the mass fraction of diacylglycerol reached 48%. In conclusion, the continuous preparation process of diacylglycerol oil catalyzed by liquid lipase can be realized by the microchannel reactor, and the method has good stability. |
