|
| γ-亚麻酸对α-亚麻酸转化为DHA的影响 |
| Effect of γ-linolenic acid on the conversion of α-linolenic acid to DHA |
| |
| DOI：10.19902/j.cnki.zgyz.1003-7969.230354 |
| 中文关键词: γ-亚麻酸 α-亚麻酸 二十二碳六烯酸 转化 |
| 英文关键词:γ-linolenic acid α-linolenic acid docosahexaenoic acid conversion |
| 基金项目:国家自然科学基金（32072145） |
| Author Name | Affiliation | | XIE Wanfei1, JIANG Shan1, WANG Chengcheng1, WANG Yuming1,2,
XUE Changhu1,2, ZHANG Tiantian1 | (1.School of Food Science and Engineering, Ocean University of China, Qingdao 266404,
Shandong，China
2.Pilot National Laboratory for Marine Science and
Technology (Qingdao), Qingdao 266237, Shandong，China) |
|
| Hits: 11 |
| Download times: 2 |
| 中文摘要: |
| 为了明确γ-亚麻酸（GLA）对α-亚麻酸（ALA）转化为二十二碳六烯酸（DHA）效率的影响，以SPF级雌性ICR小鼠为研究对象，给其喂食缺乏n-3 多不饱和脂肪酸（n-3 PUFA）饲料8周构建n-3 PUFA缺乏小鼠模型，再将其分为3组，分别为对照组、 ALA组和 ALA+GLA组，3组小鼠分别单次灌胃生理盐水、300 mg/kg ALA和300 mg/kg ALA+30 mg/kg GLA，灌胃24 h后，提取各组小鼠血清、肝脏、小肠壁、脑中脂质，测定血清和肝脏甘油三酯（TG）含量，以及血清、肝脏、小肠壁、脑脂质脂肪酸组成及DHA含量。结果表明：相比对照组，ALA组和ALA+GLA组小鼠血清TG浓度降低，ALA组小鼠肝脏TG含量增加，ALA+GLA组小鼠肝脏TG含量降低；3组小鼠血清、肝脏、小肠壁、脑脂肪酸组成均无显著差异，相比血清、肝脏，小肠壁中脂肪酸种类较多，且检出ALA；相比ALA组，ALA+GLA组小鼠肝脏和小肠壁中的DHA含量显著下降，且肝脏中DHA含量显著降低主要体现在TG型DHA的降低上，但两组小鼠脑中DHA含量以及血清DHA浓度无显著差异。综上，补充ALA可提高小鼠外周组织DHA含量，GLA对ALA向DHA的转化具有抑制作用。 |
| 英文摘要: |
| In order to determine the effect of γ-linolenic acid (GLA) on the conversion efficiency of α-linolenic acid (ALA) to docosahexaenoic acid (DHA), female ICR mice with SPF grade were fed with n-3 PUFA deficient diet for 8 weeks to establish a n-3 PUFA deficient mouse model. The mice were divided into three groups: control group, ALA group and ALA+GLA group. The mice in the three groups were given a single gavage of normal saline, 300 mg/kg ALA, 300 mg/kg ALA+30 mg/kg GLA, respectively. After 24 h of gavage, the lipids in the serum, liver, small intestine wall and brain of mice in each group were extracted. The contents of triglyceride (TG) in serum and liver, fatty acid composition and DHA content in serum, liver, small intestinal wall and brain of mice were determined. The results showed that compared with the control group, the TG content in serum of mice in ALA group and ALA+GLA group decreased, the TG content in liver of mice in ALA group increased, and the TG content in liver of mice in ALA+GLA group decreased. There was no significant difference in fatty acid composition in serum, liver, small intestinal wall and brain of mice in the three groups. Compared with serum and liver, there were more fatty acid types in small intestinal wall of mice, and ALA was detected. Compared with ALA group, the DHA content in liver and small intestinal wall of mice in ALA+GLA group significantly decreased, and the DHA content in liver significantly decreased mainly reflected in the decrease of TG-type DHA, but the DHA content in brain of mice and the DHA concentration in serum of mice in the two groups was not significantly different. In conclusion, ALA supplementation can increase the content of DHA in peripheral tissues of mice, and GLA can inhibit the conversion of ALA to DHA. |
| 查看全文 View/Add Comment Download reader |
| Close |
|
|
|
