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| Effect of γ-linolenic acid on the conversion of α-linolenic acid to DHA |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230354 |
| KeyWord:γ-linolenic acid α-linolenic acid docosahexaenoic acid conversion |
| FundProject:国家自然科学基金（32072145） |
| Author Name | Affiliation | | XIE Wanfei1, JIANG Shan1, WANG Chengcheng1, WANG Yuming1,2,
XUE Changhu1,2, ZHANG Tiantian1 | (1.School of Food Science and Engineering, Ocean University of China, Qingdao 266404,
Shandong，China
2.Pilot National Laboratory for Marine Science and
Technology (Qingdao), Qingdao 266237, Shandong，China) |
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| Abstract: |
| In order to determine the effect of γ-linolenic acid (GLA) on the conversion efficiency of α-linolenic acid (ALA) to docosahexaenoic acid (DHA), female ICR mice with SPF grade were fed with n-3 PUFA deficient diet for 8 weeks to establish a n-3 PUFA deficient mouse model. The mice were divided into three groups: control group, ALA group and ALA+GLA group. The mice in the three groups were given a single gavage of normal saline, 300 mg/kg ALA, 300 mg/kg ALA+30 mg/kg GLA, respectively. After 24 h of gavage, the lipids in the serum, liver, small intestine wall and brain of mice in each group were extracted. The contents of triglyceride (TG) in serum and liver, fatty acid composition and DHA content in serum, liver, small intestinal wall and brain of mice were determined. The results showed that compared with the control group, the TG content in serum of mice in ALA group and ALA+GLA group decreased, the TG content in liver of mice in ALA group increased, and the TG content in liver of mice in ALA+GLA group decreased. There was no significant difference in fatty acid composition in serum, liver, small intestinal wall and brain of mice in the three groups. Compared with serum and liver, there were more fatty acid types in small intestinal wall of mice, and ALA was detected. Compared with ALA group, the DHA content in liver and small intestinal wall of mice in ALA+GLA group significantly decreased, and the DHA content in liver significantly decreased mainly reflected in the decrease of TG-type DHA, but the DHA content in brain of mice and the DHA concentration in serum of mice in the two groups was not significantly different. In conclusion, ALA supplementation can increase the content of DHA in peripheral tissues of mice, and GLA can inhibit the conversion of ALA to DHA. |
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