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| Preparation of lysophospholipids through ethanolysis of soybean phospholipids catalyzed by Lipozyme TLIM |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230596 |
| KeyWord:soybean phospholipids lysophospholipids lipase ethanolysis |
| FundProject:河南省科技研发计划联合基金（应用攻关类）（222103810064） |
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| Abstract: |
| In order to improve the functional properties of soybean phospholipids and expand their applications, lysophospholipids was prepared by Lipozyme TLIM catalyzed ethanolysis of soybean phospholipids, the effects of reaction time and substrate mass concentration on ethanolysis reaction were examined by single factor test,and the optimal process conditions were obtained using response surface methodology. The results showed that the contents of phosphatidylcholine(PC), phosphatidylserine(PS) and phosphatidyl ethanolamine (PE) decreased with the extension of the reaction time, and the contents of phosphatidic acid (PA), phosphatidylinositol (PI), sphingomyelin (SM), N-acylphosphatidylethanolamine (APE), cardiolipin (CL) and phosphatidylglycerol (PG) changed slightly, and 2-lysophosphatidylcholine (2-LPC) was the primary product. The optimal conditions for Lipozyme TLIM catalyzed ethanolysis of soybean phospholipids were as follows: substrate mass concentration 1.0 g/mL, reaction temperature 20 ℃, enzyme loading amount 15% (based on the mass of soybean phospholipids), water content 2% (based on the mass of soybean phospholipids), and reaction time 24 h. Under these conditions, the content of lysophospholipids was 67.80%, and the lysophospholipids mainly consisted of 2-LPC with content of 48.45%, with small amounts of lysophosphatidic acid(LPA), lysophosphatidyl-serine (LPS), lysophosphatidylethanolamine(LPE) and so on. In the 13C-NMR spectrum of the ethanolysis product, the signal of fatty acid ethyl ester was strong and sharp, while that of fatty acid was very weak, which proved that ethanolysis was the major reaction in the Lipozyme TLIM catalyzed soybean phospholipids enzymolysis. In conclusion, the preparation of lysophospholipids by Lipozyme TLIM catalyzed ethanolysis of soybean phospholipids can provide a new way for phospholipids modification. |
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