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| Lipozyme TLIM催化大豆磷脂乙醇解制备溶血磷脂 |
| Preparation of lysophospholipids through ethanolysis of soybean phospholipids catalyzed by Lipozyme TLIM |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230596 |
| 中文关键词: 大豆磷脂 溶血磷脂 脂肪酶 乙醇解 |
| 英文关键词:soybean phospholipids lysophospholipids lipase ethanolysis |
| 基金项目:河南省科技研发计划联合基金（应用攻关类）（222103810064） |
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| 中文摘要: |
| 为改善大豆磷脂的功能特性，扩大其应用范围，采用Lipozyme TLIM催化大豆磷脂乙醇解制备溶血磷脂，通过单因素实验考察了反应时间和底物质量浓度对乙醇解反应的影响，并通过响应面实验优化其工艺条件。结果表明：在大豆磷脂乙醇解反应过程中，随着反应时间的延长，磷脂酰胆碱（PC）、磷脂酰丝氨酸（PS）、磷脂酰乙醇胺（PE）的含量不断降低，磷脂酸（PA）、磷脂酰肌醇（PI）、鞘磷脂（SM）、N-酰基磷脂酰乙醇胺（APE）、心磷脂（CL）、磷脂酰甘油（PG）含量变化较小，主要生成2-溶血磷脂酰胆碱（2-LPC）；Lipozyme TLIM催化大豆磷脂乙醇解的最佳工艺条件为底物质量浓度1.0 g/mL、反应温度20 ℃、加酶量15%（以大豆磷脂质量计）、加水量2%（以大豆磷脂质量计）、反应时间24 h，在此条件下溶血磷脂含量为67.80%，且溶血磷脂主要以2-LPC为主,含量为48.45%，还含有少量的溶血磷脂酸（LPA）、溶血磷脂酰丝氨酸（LPS）、溶血磷脂酰乙醇胺（LPE）等，在乙醇解产物13C-NMR图谱中，脂肪酸乙酯有强而尖锐的信号，脂肪酸的信号极弱，证明Lipozyme TLIM催化大豆磷脂醇解过程中主要发生了乙醇解反应。综上，利用脂肪酶乙醇解大豆磷脂制备溶血磷脂为大豆磷脂的改性拓宽了新思路。 |
| 英文摘要: |
| In order to improve the functional properties of soybean phospholipids and expand their applications, lysophospholipids was prepared by Lipozyme TLIM catalyzed ethanolysis of soybean phospholipids, the effects of reaction time and substrate mass concentration on ethanolysis reaction were examined by single factor test,and the optimal process conditions were obtained using response surface methodology. The results showed that the contents of phosphatidylcholine(PC), phosphatidylserine(PS) and phosphatidyl ethanolamine (PE) decreased with the extension of the reaction time, and the contents of phosphatidic acid (PA), phosphatidylinositol (PI), sphingomyelin (SM), N-acylphosphatidylethanolamine (APE), cardiolipin (CL) and phosphatidylglycerol (PG) changed slightly, and 2-lysophosphatidylcholine (2-LPC) was the primary product. The optimal conditions for Lipozyme TLIM catalyzed ethanolysis of soybean phospholipids were as follows: substrate mass concentration 1.0 g/mL, reaction temperature 20 ℃, enzyme loading amount 15% (based on the mass of soybean phospholipids), water content 2% (based on the mass of soybean phospholipids), and reaction time 24 h. Under these conditions, the content of lysophospholipids was 67.80%, and the lysophospholipids mainly consisted of 2-LPC with content of 48.45%, with small amounts of lysophosphatidic acid(LPA), lysophosphatidyl-serine (LPS), lysophosphatidylethanolamine(LPE) and so on. In the 13C-NMR spectrum of the ethanolysis product, the signal of fatty acid ethyl ester was strong and sharp, while that of fatty acid was very weak, which proved that ethanolysis was the major reaction in the Lipozyme TLIM catalyzed soybean phospholipids enzymolysis. In conclusion, the preparation of lysophospholipids by Lipozyme TLIM catalyzed ethanolysis of soybean phospholipids can provide a new way for phospholipids modification. |
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