|
| Simultaneous determination of four vitamin E isomers in vegetable oil by ultra-high performance liquid chromatography- triple quadrupole tandem mass spectrometry |
| |
| DOI：10.19902/j.cnki.zgyz.1003-7969.230358 |
| KeyWord:vitamin E vegetable oil UPLC-MS/MS |
| FundProject:未来食品技术创新中心建设项目资金(BM2020023) |
| Author Name | Affiliation | | LI Cailing1, ZHANG Jian1,2, LIU Yuanfa1,3, WANG Xiaochao1, MEI Dongxu1 | （1.Future Food (Bai Ma) Research Institute, Nanjing 210000，China
2.Nanjing Fuzhe Future Food
Research Institute Co. , Ltd. , Nanjing 210000, China
3.School of Food Science and Technology, Jiangnan
University, Wuxi 214122, Jiangsu, China） |
|
| Hits: 584 |
| Download times: 589 |
| Abstract: |
| To improve the detection efficiency of four vitamin E isomers (α-, β-, γ-, δ - tocopherol) in vegetable oil, a method for simultaneous determination of the content of four vitamin E isomers (α-, β-, γ-, δ - tocopherol) in vegetable oil by ultra-high performance liquid chromatography-triple quadrupole tandem mass spectrometry (UPLC-MS/MS) was established. The conditions of mass spectrometry, chromatography, and sample pretreatment were optimized, and the method established was examined. The results showed that the sample (0.5 g) was extracted with methanol(10 mL), purified with C18 powder （35 mg）, separated with Kintex F5 chromatographic column (2.1 mm×100 mm, 1.7 μm), eluted with 0.1% formic acid aqueous solution and 0.1% formic acid-methanol solution as mobile phase by gradient under the speed of 0.4 mL/min, detected under positive ion mode of electric spray (ESI) and multi reaction monitoring mode (MRM), and quantified by external standard method. Under these conditions, there were a good linear relationships for four vitamin E isomers in the mass concentration range of 1-100 ng/mL, with correlation coefficients (R2) above 0.998, detection limits of 6 ng/g, and quantification limits of 20 ng/g. The recovery rate ranged from 92.0% to 108.0%, the relative standard deviation was 0.7%-7.1%, and the relative error of the determination results with national standard method was -4.3%-5.1%. This method has the advantages of accuracy, sensitivity, and good reproducibility, and it can be applied to the analysis of actual samples. |
| View full text View/Add Comment Download reader |
| Close |
|
|
|
