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| Response surface optimization of the extraction process conditions for sesame protein using choline chloride-glycerol system |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230679 |
| KeyWord:deep eutectic solvent sesame meal sesame protein choline chloride functional characteristics secondary structure |
| FundProject:国家现代农业产业技术体系资助（CARS-14-1-29，CARS-14-1-01） |
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| Abstract: |
| To improve the comprehensive utilization rate of protein in sesame cake and meal, a by-product of sesame oil, sesame protein was extracted from sesame meal using choline chloride -glycerol (molar ratio 1∶ 3) system (deep eutectic solvent, DES), the extraction process conditions were optimized through single factor experiments and response surface methodology, and the functional characteristics of sesame protein extracted under the optimal conditions were analyzed. Fourier transform infrared spectroscopy and scanning electron microscopy were used to characterize the structure of sesame protein. In addition, the sesame protein extracted by DES method was compared with that by alkali solution-acid precipitation method. The results showed that the optimal conditions for extracting sesame protein were as follows: moisture content in DES 10%, solid-liquid mass ratio 1∶ 29.4, extraction temperature 59.0 ℃, extraction time 1 h, and stirring speed 750 r/min. Under these conditions, the extraction rate of sesame protein was (64.65 ±0.52)%, significantly lower than that of the alkali solution-acid precipitation method((74.19±1.79)%). The solubility, oil absorption, foam stability and emulsification stability of sesame protein extracted by DES were significantly higher than those extracted by alkali solution-acid precipitation method, while the water absorption was significantly lower than that extracted by alkali solution-acid precipitation method. The β- folding contents of the protein obtained by DES was significantly lower than that obtained by alkali solution-acid precipitation method, while the contents of other secondary structures (α-helix, aperiodical coil, β-turn angle, β-inverse folding) were significantly higher than those obtained by alkali solution-acid precipitation method. In conclusion, the DES extraction method can be used as a new green sesame protein extraction method. |
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