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| Preparation process optimization of hemp kernel antithrombotic active peptide |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230357 |
| KeyWord:hemp kernel antithrombotic active peptide enzymatic hydrolysis response surface methodology thrombin inhibition rate |
| FundProject:黑龙江省省属科研院所科研业务费项目(CZKYF2022-1-C004)；黑龙江省科学院重大攻关项目（ZDGG2024DQ01） |
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| Abstract: |
| In order to obtain hemp kernel polypeptide with antithrombotic activity, hemp kernel protein powder was used as raw material to obtain hemp kernel antithrombotic active peptide by enzymatic hydrolysis, and it was purified by ultrafiltration. The enzymatic hydrolysis process was optimized by single factor experiment and response surface methodology, and the molecular weight distribution of hemp kernel antithrombotic active peptide purified were identified. The results showed that the optimal conditions for the enzymatic hydrolysis process were obtained as follows: using trypsin, enzymatic hydrolysis temperature 40 ℃, solid-liquid ratio 1∶ 25, enzyme dosage 5.68%, and pH 8.0. Under these conditions, the thrombin inhibition rate was 84.70% when the enzymatic hydrolysis time was 5 h. The hemp kernel antithrombotic active peptide with a molecular weight below 3 000 Da had a stronger thrombin inhibition rate at a mass concentration of 0.01 g/mL, which could reach 99.38%. Nearly 70% of the peptides with a molecular weight below 3 000 Da were peptides with a molecular weight of 500-1 500 Da. In summary, the hemp kernel antithrombotic active peptide prepared and purified by this method has high thrombin inhibitory activity. |
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