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| Effect of hemp kernel polypeptide on reducing uric acid in zebrafish with hyperuricemia and analysis of its amino acid sequence |
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| DOI： |
| KeyWord:hemp kernel polypeptide zebrafish hyperuricemia uric acid-lowering amino acid sequence |
| FundProject:功能食品工程技术领军人才梯队建设项目（RC2022DQ01）；黑龙江省科学院对外合作项目（HZ2022-04）； 黑龙江省科学院院长基金项目（YZ2023DQ01）；黑龙江省省属院所科研业务费项目（CZKYF2021-2-B015） |
| Author Name | Affiliation | | WEI Lianhui, DONG Yan, SHI Jie, LI Guowei, ZHANG Zhenghai,
JI Yanru, YANG Qingli, PAN Jing | Daqing Branch of the Heilongjiang Academy of Sciences, Daqing 163319, Heilongjiang, China |
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| Abstract: |
| In order to promote the application of hemp kernel polypeptide in functional foods and specialty medical foods, the uric acid-lowering effect and its possible mechanisms of hemp kernel polypeptide in zebrafish model were investigated, and the amino acid sequences of polypeptide components with uric acid-lowering activity were analyzed. The zebrafish hyperuricemia model was established by feeding zebrafish with an uricase inhibitor potassium oxonate at 10 mmol/L and uric acid synthesis precursor xanthine sodium salt at 50 μmol/L for 20 h. Hemp kernel polypeptide with different mass concentrations administered to the zebrafish, with allopurinol as a positive control. The fluorescence intensity of uric acid, and mRNA expression of hypoxanthine guanine-phosphoribosyltransferase(HPRT1) and organic anion transporter(OAT1) gene in zebrafish were measured. The amino acid sequences of the active polypeptides were analyzed by liquid chromatography-mass spectrometry. The results showed that hemp kernel polypeptide effectively reduced uric acid level of zebrafish through up-regulating HPRT1 and OAT1 gene expression. The molecular weight of eight polypeptides with high abundance in hemp kernel polypeptide were 499.23, 578.24, 628.27, 653.29, 715.31, 918.43, 982.41, 1 128.49 Da, and their amino acid sequences were PGTPE, MPDDV, TTSYTG, TPHWN, NNGDSPL, DDFNPRR, NPHDEFQP, YHSYLCKTD, respectively. In conclusion, the hemp kernel polypeptide can be used as functional components in the development of functional food and health care products related to lowering uric acid. |
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