DHA-phospholipid (DHA-PL) has attracted much attention in recent years because of its high absorption rate of DHA. Aiming to provide reference for the application of DHA-PL in functional lipid products, the enzymatic preparation method, detection method and functional characteristics of DHA-PL were summarized. Enzymatic preparation of DHA-PL had the advantages of mild conditions and high safety, and mainly divided into enzyme catalytic acid hydrolysis method, enzyme catalytic transesterification method and enzyme catalytic esterification method. The methods for detecting DHA-PL include thin layer chromatography-gas chromatography, nuclear magnetic resonance, high performance liquid chromatography-tandem mass spectrometry, etc. DHA-PL has the functions of anticancer, improving lipid metabolism, anti-inflammatory and enhancing neurotrophic activity. Optimization of the preparation process and improvement of detection methods for DHA-PL is of great significance for the application of DHA-PL in industries such as food and pharmaceuticals. |