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| Preparation of medium- and long- chain triglycerides by enzymatic transesterification of high oleic acid sunflower seed oil |
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| DOI： |
| KeyWord:medium-and long-chain triglycerides high oleic acid sunflower seed oil coconut oil transesterification response surface method |
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| Abstract: |
| In order to provide references for the development of healthy fats and oils, the medium-and long-chain triglycerides (MLCT) was synthesized from high oleic acid sunflower seed oil and coconut oil catalyzed by the lipase Lipozyme RM IM, and the synthesis conditions of MLCT were optimized using single factor test and response surface test. The fatty acid compositions of the raw oils were compared with the product produced under the optimal process conditions. The results showed that the optimal MLCT synthesis conditions were reaction temperature 61 ℃, reaction time 6.2 h, enzyme dosage 9.3%, and substrate ratio 6∶ 4 (molar ratio of coconut oil to high oleic acid sunflower seed oil). Under the optimal conditions, the MLCT content was 75.98%, and the main fatty acids in the product were oleic acid (42.711%) and lauric acid (26.226%). In conclusion, the prepared product is rich in MLCT and has a large potential for market application. |
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