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| Hypotensive effect in vivo of hemp protein peptides |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240390 |
| KeyWord:hemp seed protein hemp protein peptides SHR hypotensive effect heart rate body weight long-acting short-acting |
| FundProject:国家自然科学基金项目(32160560)；广西自然科学基金项目（2024GXNSFAA010336，2016GXNSFBA380167） |
| Author Name | Affiliation | | WEI Ping1,2, LIU Guoming2,3, WEI Zhen1, DENG Zhonglin1 | 1.Agro-food Science and Technology Research Institute, Guangxi Academy of Agricultural
Sciences, Nanning 530007, China
2.Guangxi Key Laboratory of Fruits and Vegetables
Storage-processing Technology, Nanning 530007, China
3.Guangxi Academy of
Agricultural Sciences, Nanning 530007, China |
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| Abstract: |
| In order to provide a theoretical basis for the use of hemp seed protein as a functional factor for lowering blood pressure, hemp seed protein was prepared by the alkali-soluble and acid-precipitation method with hemp kernel as materials, hemp protein peptides were prepared through a stepwise enzymatic hydrolysis process, and then hemp protein peptides were used as raw material to explore its hypotensive effect in vivo in terms of animal experiments. A normal control group (male Wistar rats), positive control group (captopril 20 mg/kg, male spontaneously hypertensive rats (SHR)), hemp protein peptides medium dose group (750 mg/kg, male SHR) and hemp protein peptides high dose group (1 500 mg/kg, male SHR) were set up to study the effect of short-term (once daily) and long-term (21 d consecutively) administration on the rat tail artery systolic blood pressure (SBP), heart rate and body weight. The free amino acid composition of hemp protein peptides was analyzed. The results showed that after adminstation with captopril and hemp protein peptides for 1 h of single day and 7 d of continuous administration, the SBP of the SHR could be significantly reduced, and the hypotensive effect of short-term administration and long-term administration could last for 5 h and 21 d, respectively. There was no significant hypotensive difference between medium/high dose group and captopril group （p>0.05）. Short-term and long-term administration both had a certain impact on the heart rate of SHR, while long-term administration did not significantly affect the body weight of SHR （p>0.05）. The proportion of hydrophobic amino acids in hemp protein peptides that are related to the effect of lowering blood pressure was as high as 31.91%. In addition, the contents of some aromatic amino acid(Phe), aliphatic amino acid(Leu and Val), basic amino acids (Arg and Lys) were also relatively high. In summary, the hemp protein peptides has a certain hypotensive effect in SHR, and is expected to be a new functional ingredient for lowering blood pressure. |
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