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| Isolation and antioxidant activity analysis of condensed tannin polymers in walnut pellicle |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230612 |
| KeyWord:walnut pellicle condensed tannins polymer antioxidant activity |
| FundProject:中央级公益性科研院所基本科研业务费专项资金（CAFYBB2019QD002） |
| Author Name | Affiliation | | CUI Maokai1,2, HUANG Haitao3, LI Qingyang1, SHEN Danyu1,
XIAO Pengfei3, LIU Yihua1 | 1.Research Institute of Subtropical Forestry, Chinese Academy of Forestry, Hangzhou 311400, China
2.School of Landscape Architecture, Nanjing Forestry University, Nanjing 210037, China
3.School of
Biology and Environment, Jiyang College of Zhejiang Agriculture and Forestry University, Shaoxing
312000, Zhejiang, China |
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| Abstract: |
| To provide a reference for the high-value utilization of walnut resources, walnut pellicle was used as raw material and extracted with a 70% methanol solution (containing 0.5% acetic acid). The extract was separated with a Sephadex LH-20 dextran gel column and subjected to gradient elution with different volume fractions of methanol solution to obtain 13 fractions of condensed tannin polymers (F1-F13). The in vitro antioxidant activity of these 13 fractions were analyzed using vitamin C as the standard sample. The results indicated that the gradient elution method with methanol solution (volume fraction 40%-75%) could successfully separate condensed tannin dimers (DP2) to heptamers (DP7) from walnut pellicle. The elution volume fractions for condensed DP2 and trimers (DP3) ranged from 40% to 60%, while the elution volume fractions for tetramers (DP4) and three other higher polymers were between 60% and 75%. After optimizing the elution process, efficient separation of DP2 and DP3 was achieved, with the elution rate of DP2 exceeding 80%. DP2 was isolated from elution fractions F2, F3, and F4, with a tatal content exceeding 1.40 mg/g, while DP3 was obtained from fractions F5 and F6, with contents of 0.66 mg/g and 0.59 mg/g, respectively. All fractions exhibited good antioxidant activity, with F4, primarily containing DP2, showing the highest antioxidant activity, measured by ferric ion reducing ant ioxidant power (FRAP) and DPPH methods as (17.22±0.68) mg/g and (24.36±0.73) mg/g, respectively. In conclusion, the gradient elution method enables the effective separation of condensed tannin polymers, allowing for the isolation of DP2 and DP3 from walnut pellicle. |
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