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| Breeding of Mortierella alpina mutants with high yield of arachidonic acid by UV-LiCl combined with ARTP mutagenesis |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240040 |
| KeyWord:arachidonic acid Mortierella alpina mutagenesis breeding UV-LiCl ARTP |
| FundProject:辽宁省自然科学基金（2024-MS-171） |
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| Abstract: |
| In order to obtain a genetically stable and high yield arachidonic acid (ARA)-producing Mortierella alpina strain, mutagenesis of the strain of M. alpina FD-1 preserved in laboratory was performed by UV-LiCl combined with atmospheric and room temperature plasma (ARTP) method, and the strain was screened by TTC plate and the fermentation in shaking flasks. The results showed that a mutant strain Z6-A23 with high yield of ARA was obtained by UV-LiCl combined with ARTP mutagenesis. The ARA content in the oil produced by the mutant strain Z6-A23 reached as high as 44.57%, and the ARA yield was 3.62 g/L, which were 75.40% and 223.21% higher than those of the original strain FD-1, respectively. After 10 subcultures, the mutant Z6-A23 had stable ability to synthesize ARA, indicating that it had good genetic stability. In conculsion, the mutant strain Z6-A23 obtained is expected to be a promising strain for efficient ARA production. |
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