Optimization of extraction process for high α-linolenic acid perilla seed oil using high speed shear assisted aqueous enzymatic method
  
DOI:10.19902/j.cnki.zgyz.1003-7969.240044
KeyWord:perilla seed oil  α-linolenic acid  high speed shearing  aqueous enzymatic method  response surface experiment
FundProject:甘肃省市场监督管理局科技计划项目(SSCJG-SP-202110)
Author NameAffiliation
RUI Wenjun, SUN Shikun, FU Lei, SUN Yan, LIU Yu Gansu Food Inspection Research Institute, Lanzhou 730030, China 
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Abstract:
      In order to provide technical guidance for the production of perilla seed oil, high speed shear assisted aqueous enzymatic method was used to extract perilla seed oil. With α-linolenic acid yield as response value, solid-liquid ratio, shear rate, shear time, enzyme treatment temperature and enzyme treatment time as independent variables, the extraction process of perilla seed oil was optimized by single factor experiment and response surface experiment. The results showed that the optimum conditions for extracting perilla seed oil by high speed shear assisted aqueous enzymatic method were as follows: solid-liquid ratio 1∶ 4, shear rate 12 100 r/min, shear time 4.2 min, enzyme treatment temperature 40 ℃, and enzyme treatment time 3 h. Under the optimum conditions, the yield of α-linolenic acid was 38.2%. In conclusion, the high speed shear assisted aqueous enzymatic method can effectively extract perilla seed oil, and the yield of α-linolenic acid is higher.
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