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Optimization of extraction process for high α-linolenic acid perilla seed oil using high speed shear assisted aqueous enzymatic method |
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DOI:10.19902/j.cnki.zgyz.1003-7969.240044 |
KeyWord:perilla seed oil α-linolenic acid high speed shearing aqueous enzymatic method response surface experiment |
FundProject:甘肃省市场监督管理局科技计划项目(SSCJG-SP-202110) |
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Abstract: |
In order to provide technical guidance for the production of perilla seed oil, high speed shear assisted aqueous enzymatic method was used to extract perilla seed oil. With α-linolenic acid yield as response value, solid-liquid ratio, shear rate, shear time, enzyme treatment temperature and enzyme treatment time as independent variables, the extraction process of perilla seed oil was optimized by single factor experiment and response surface experiment. The results showed that the optimum conditions for extracting perilla seed oil by high speed shear assisted aqueous enzymatic method were as follows: solid-liquid ratio 1∶ 4, shear rate 12 100 r/min, shear time 4.2 min, enzyme treatment temperature 40 ℃, and enzyme treatment time 3 h. Under the optimum conditions, the yield of α-linolenic acid was 38.2%. In conclusion, the high speed shear assisted aqueous enzymatic method can effectively extract perilla seed oil, and the yield of α-linolenic acid is higher. |
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