| In order to provide a reference for the development and utilization of Antarctic krill oil, with Antarctic krill powder as material, the neutral lipids were extracted by supercritical CO2 and then polar lipids were extracted by ethanol. The extraction process of different polar lipids in Antarctic krill oil were optimized by single factor experiment and response surface methodology, and the components of the two lipids were determined. The results showed that the optimal extraction conditions of neutral lipids by the combination of supercritical CO2 were extraction pressure 45 MPa, extraction temperature 45 ℃ and extraction time 1.75 h. Under these conditions, the content of triglyceride in neutral lipids was 73.68%, the contents of astaxanthin, tocopherol and vitamin A were 74.91, 44.96 mg/kg and 448.31 mg/kg, respectively, and the extraction rate of lipids was 40.81%. The optimal extraction conditions of polar lipids by ethanol were solid-liquid ratio 1∶ 3, extraction temperature 57 ℃ and extraction time 3 h. Under these conditions, the content of phospholipid in polar lipids was 80.19%, the contents of EPA and DHA were 20.74% and 11.43%, respectively, and the extraction rate of lipids was 58.37%.In conclusion, the combination of supercritical CO2 extraction and ethanol extraction can effectively separate neutral lipids and polar lipids in Antarctic krill oil. |
