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| Preparation of rapeseed oil rich in nervonic acid by enzymatic transesterification and its quality analysis |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240170 |
| KeyWord:rapeseed oil nervonic acid lipase transesterification |
| FundProject:陕西省重点研发一般项目（2024NC-YBXM-135）；陕西省科技创新团队（2024RS-CXTD-70） |
| Author Name | Affiliation | | ZHU Jiabin, WANG Yuanyuan, MA Gaiqin, LI Yuefan,
LI Qi, GAO Yuan, YU Xiuzhu | Shaanxi "Four Subjects and One Union" Engineering Technology School-Enterprise Joint Research Center of
Functional Oils, College of Food Science and Engineering, Northwest A & F University,
Yangling 712100, Shaanxi, China |
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| Abstract: |
| In order to provide a reference for the preparation of rapeseed oil rich in nervonic acid products that are easily absorbed by human body in the actual production and processing, the rapeseed oil rich in nervonic acid was prepared by enzymatic transesterification with nervonic acid ethyl ester and rapeseed oil as materials. The enzymatic transesterification reaction conditions were optimized by single factor experiment with the nervonic acid content in the product and transesterification rate as indicators. The change of total and sn-2 fatty acid composition, acid value, peroxide value, K232, K268, and phytosterol and squalene contents of the rapeseed oil before and after transesterification were determined. The results showed that the optimal transeseterification conditions were as follows: mass ratio of nervonic acid ethyl ester to rapeseed oil 1∶ 10, Novozym 435 lipase dosage 8%(on the basis of substrates mass), reaction temperature 60 ℃, and reaction time24 h. Under the optimal conditions, the nervonic acid content in the prepared rapeseed oil was up to 11.11% , the nervonic acid content in sn-2 position was up to 8.92%, and the transesterification yield reached 96.61%. After the transesterification, the values of K232,K268 of rapeseed oil increased significantly. However, the acid value and peroxide value met the requirement of GB/T 1536-2021. Moreover, except for stigmasterol, the contents of campesterol, brassicasterol, β-sitosterol, and squalene decreased significantly. In conclusion, it is feasible to prepare rapeseed oil rich in nervonic acid through enzymatic transesterification, but it is necessary to closely monitor the changes in oil quality indicators during the reaction process. |
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