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| Comparison of the memory-improving effects of two refined fish oils with different DHA contents on mice |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240107 |
| KeyWord:fish oil docosahexaenoic acid (DHA) improving memory jumping test dark avoidance test water maze test |
| FundProject:湖南省药物非临床研究科技创新创业团队（2021） |
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| Abstract: |
| To provide a reference for the clinical application of fish oil, refined fish oils with DHA-labeled contents of 85% and 40% were used as test samples, female ICR mice were randomly divided into the following groups: a negative control (NC) group, a normal group with low-, medium-, and high-doses of DHA-labeled fish oil (85% and 40%) at 0.2, 0.4 g/kg, and 0.6 g/kg, a model control (MC) group, a model group with low-, medium-, and high-doses of DHA-labeled fish oil (85% and 40%) at 0.2, 0.4 g/kg, and 0.6 g/kg, and the mice were administered 5 mL/kg of the fish oil orally once a day for 30 d. After the last administration, 10 mice from the NC group and each dose normal group were directly subjected to the jumping test, dark avoidance test, and water maze test, while the model groups were tested with jumping test, dark avoidance test, and water maze test after intraperitoneal injection of scopolamine to induce the model. The results showed that compared with the NC group, the high-dose normal group of fish oil (DHA 85% and 40%) had significantly longer retest phase latency in the jumping test (p≤0.05), and the medium-dose normal group of fish oil (DHA 85%) and the high-dose normal group of fish oil (DHA 40%) had significantly longer extinction phase latency in dark avoidance test (p≤0.05). Compared with MC group, the medium-dose model group of fish oil (DHA 85%) had significantly longer retest phase latency (p≤0.05) and a significantly lower error rate of animals (p≤0.05) in the jumping test, the low-dose model group of fish oil (DHA 85%) had significantly longer retest and extinction phase latency in the dark avoidance test (p≤0.05), the errors number of the high-dose model group of fish oil (DHA 40%) in the dark avoidance test training stage was significantly reduced (p≤0.05). In the water maze test, compared with MC group, the total time to reach the end in the low-, medium-, and high-dose model groups of fish oil (DHA 85%) and fish oil (DHA 40%) at the training and test stage decreased significantly (p≤0.05 or p≤0.01), the total number of errors at the training and test stage in the medium- and high-dose model groups of fish oil (DHA 85%) and the high-dose model groups of fish oil (DHA 40%) reduced significantly (p≤0.05), and the proportion of the total number of animals reaching the end at the training and test stage in the low-, medium- and high-dose model groups of fish oil (DHA 85%) and the medium-dose model groups of fish oil (DHA 40%) increased significantly (p≤0.05 or p≤0.01). In the model group, the latency of medium-dose fish oil (DHA 85%) in the retest stage of jumping test was significantly longer than that in the high-dose of DHA 40%(p≤0.05), and the proportion of mice with false response was significantly lower than that in the high-dose of fish oil (DHA 40%) (p≤0.05), and the latency of low-dose of fish oil (DHA 85%) in dark avoidance test was significantly longer than that of high dose of fish oil (DHA 40%)(p≤0.05). In summary, fish oil (DHA 85%) and fish oil (DHA 40%) can improve the memory of mice, and the effect of fish oil (DHA 85%) is better than that of fish oil (DHA 40%). |
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