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| Identification of phospholipids in pasteurized milk using SPE-HILIC-QTOF-MS |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240058 |
| KeyWord:phospholipid solid-phase extraction hydrophilic interaction liquid chromatography-quadrupole-time-of-flight-mass spectrometry |
| FundProject:“十四五”国家重点研发计划重点专项(2022YFF0606800)；中国农业科学院基本科研业务费专项（1610072022007，Y2023PT19） |
| Author Name | Affiliation | | GU Jingyi, LI Xiabing, LIAO Guangqin, WANG Tiancai, WANG Zishuang,
JIA Qi, ZHANG Xinglian, QIAN Yongzhong, QIU Jing | Key Laboratory for Quality and Safety of Agricultural Products of the Ministry of Agriculture and Rural Affairs,
Institute of Agricultural Quality Standards and Testing Technology，Chinese Academy of Agricultural
Sciences, Beijing 100081，China |
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| Abstract: |
| In order to comprehensively understand of pasteurized milk phospholipids composition and provide a data base for milk phospholipid research, a solid-phase extraction (SPE) combined with hydrophilic interaction liquid chromatography (HILIC)-quadrupole- time-of-flight (QTOF)-mass spectrometry (MS) technique was developed for the identification of phospholipids in pasteurized milk. Lipid extraction, SPE, and MS conditions were optimized and the phospholipid composition of pasteurized milk from four mammalian species (camel, goat, buffalo and cow) was analyzed. The results showed that 4 mL of dichloromethane-methanol （volume ratio 2∶ 1） was selected to extract the lipid from 1 mL of the sample, and the phospholipid fractions were separated by a two-step washing of 10 mL of n-hexane and 10 mL of isopropanol-dichloromethane （volume ratio 3∶ 7） with a one-step elution of 2 mL of methanol; three iterations were selected to collect the MS/MS information of the sample. A total of 580 phospholipid molecules covering 7 subclasses were identified, including 116 sphingomyelins, 18 cardiolipins, 181 phosphatidylethanolamines, 262 phosphatidylcholines, 1 phosphatidylglycerol, 1 phosphatidylinositol and 1 phosphatidylserine. In conclusion, the SPE-HILIC-QTOF-MS analytical method can effectively identify the composition of phospholipids in dairy products. |
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