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| Determination of fatty acid composition of edible vegetable oil by gas chromatography based on DB-FastFAME column |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240029 |
| KeyWord:fatty acid composition DB-FastFAME column resolution precision accuracy |
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| Abstract: |
| In order to meet the requirements of timeliness and accuracy in the detection of fatty acid composition in edible vegetable oil production enterprises, the fatty acid composition of edible vegetable oil was determined by gas chromatography method with highly polar DB-FastFAME column. The method resolution, precision, accuracy and column efficiency were analyzed. The results showed that the DB-FastFAME column could separate 37 fatty acid methyl esters mixed standard within 25 min, and the resolutions were all no less than 1.5, the relative standard deviation (RSD) was less than 0.03% for qualitation and less than 2.3% for quantitation, which indicated that the column had good separation effect and good stability. The fatty acid composition of rapeseed oil quality control sample was determined by this method, and the relative error ranged from -2.50% to 9.01%, which was unable to separate trans C18∶ 3, and the accuracy of the fatty acids was good, except for the measured value of C18∶ 3n-3 and C20∶ 1 being high. After using for 1 year, the column efficiency of DB-FastFAME column decreased and the retention time shifted by 0.9 min. In conclusion, DB-FastFAME column can meet the enterprises requirements of low-cost and high-efficiency fatty acid composition analysis, but the long-term use should be concerned about the column efficiency. |
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