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| Determination of stigmasterol, campesterol and β-sitosterol in corn sterols by high performance liquid chromatography |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240202 |
| KeyWord:corn sterols stigmasterol campesterol β-sitosterol HPLC |
| FundProject:国家“十三五”重点研发基金资助项目（2018YFD0401100） |
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| Abstract: |
| Aiming to lay the foundation for the actual production or deep exploitation and utilization of corn sterols, a high performance liquid chromatography (HPLC) method was established for the simultaneous determination of stigmasterol, campesterol and β-sitosterol in corn sterols. The separation was performed on a Waters Symmetry C18 column (4.6 mm×250 mm, 5 μm) with a diode array detector at a detection wavelength of 205 nm, and methanol-water (volume ratio 92∶ 8) as the mobile phase, and the analytes was quantified by external standard method. The results showed that the linearity of the three sterol monomers was good in the range of 125-2 000 μg/mL, and the detection limits were not more than 0.07 μg/mL for all of them. The relative standard deviations (RSD) of the peak area and retention time of the three sterol monomers ranged from 0.24% to 1.23% in the instrumental precision experiments, from 0.61% to 2.38% in the sample reproducibility experiments, from 0.13% to 2.92% in the intra-day stability experiments, and from 0.67% to 3.43% in the inter-day stability experiments. The recoveries of the three sterol monomers ranged from 76.86% to 105.74%, and the RSD were less than 5%. In conclusion, the method is characterized by high sensitivity, good accuracy, easy operation and good stability, and can realize the quantitative analysis of stigmasterol, campesterol and β-sitosterol in corn sterols. |
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