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| Fast analysis of fat-soluble vitamins A, D, E and K in milk |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240173 |
| KeyWord:ultra-high performance liquid chromatography human milk fat-soluble vitamins mammalian milk infant formula |
| FundProject: |
| Author Name | Affiliation | | HU Shaohua, ZHANG Xinghe, LU Yongmao, TAO Guanjun, WEI Wei,
JIN Qingzhe, WANG Xingguo | （School of Food Science and Technology, Jiangnan University, Wuxi 214122, Jiangsu, China) |
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| Abstract: |
| To provide reference data for determining the nutritional needs of lactating mothers and designing infant formula, a method for analyzing the contents of fat-soluble vitamins A, D, E, and K in human milk using ultra-high performance liquid chromatography (UPLC) coupled with a photodiode array detector (PDA) was established, and the method was validated. The UPLC-PDA method was applied to detect the contents of vitamins A, D, E, and K in human milk, other mammalian milks(cow milk, goat milk, and donkey milk) and infant formula. A principal component analysis (PCA) of the fat-soluble vitamin contents in different samples were also performed. The results indicated that the established UPLC-PDA method achieved separation of different fat-soluble vitamins within 10 min. The regression equations of the standard curves for each fat-soluble vitamin showed good linearity (R2≥0.999 4), with detection limits and quantification limits ranging from 0.01 μg/mL to 0.40 μg/mL and 0.09 μg/mL to 1.38 μg/mL, respectively. The calculation resluts for precision and accuracy were not greater than 5%. Except for vitamin K, which was affected by the alkaline pretreatment resulting in a lower spiked recovery rate, the spiked recovery rates for the other fat-soluble vitamins ranged from 80.69% to 111.66%, indicating good levels. Vitamin A content in human milk was between that of goat milk and cow milk, and the vitamin E content was higher than that in other mammalian milks, but significantly lower than that in infant formula. Vitamin D was not detected in human milk or other mammalian milks. PCA results showed that the composition and content of fat-soluble vitamins in mammalian milks, especially goat milk, was similar to that in human milk. In conclusion, UPLC-PDA method is suitable for analyzing the content of fat-soluble vitamins in milk and infant formula. |
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