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| Isolation and structural identification of cholesterol from egg yolk |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.230567 |
| KeyWord:egg yolk oil cholesterol isolation nuclear magnetic resonance structural identification |
| FundProject:国家自然科学基金项目（31370802）；福建省自然科学基金项目（2021J01169）；福州市农业科技项目（2021-N-210） |
| Author Name | Affiliation | | WU Huiting, XU Xianmin, WANG Yuling, JIA Wenjing,
ZHENG Yongbiao, HUANG Jianzhong, SHU Zhengyu | National and Local Joint Engineering Research Center for Industrial Microbial Fermentation Technology,
College of Life Science, Fujian Normal University, Fuzhou 350117, China |
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| Abstract: |
| In order to explore a fast and simple process for the extraction and separation of cholesterol from egg yolk, the oil in egg yolk was extracted with a binary organic solvent (ethanol-n-hexane mixed solvent), and the cholesterol content was used as an index, the oil extraction conditions were optimized by single factor experiment. The cholesterol in the egg yolk oil was isolated and purified using silica gel column chromatography, and its structural identification was carried out by nuclear magnetic resonance (NMR). The results showed that the optimal extraction conditions for egg yolk oil were volume ratio of ethanol to n-hexane 1∶ 1 and liquid to material ratio 3∶ 1. Under these conditions, the cholesterol content was 21.14 mg/g, and the extraction rate was 100%. A single component was obtained from the egg yolk oil after gradient elution on a silica gel column,and its molecular structure was initially identified as a steroidal parent core sterol composed of 27 carbon atoms by nuclear magnetic resonance hydrogen spectroscopy (1H NMR) and carbon spectroscopy (13C NMR). In conclusion, the binary organic solvent extraction combined with silica gel column chromatography can rapidly prepare cholesterol from egg yolk. |
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