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| Analysis of phospholipids and their fatty acid composition in the Hippocampus abdominalis |
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| DOI：10.19902/j.cnki.zgyz.1003-7969.240232 |
| KeyWord:Hippocampus abdominalis phospholipids fatty acid |
| FundProject:国家重点研发计划（2023YFF1103900） |
| Author Name | Affiliation | | WU Juanjuan1, TIAN Jianing1, CHEN Yimeng1, WANG Chengcheng1,
WANG Yuming1,2, JIANG Xiaoming1, XUE Changhu1, ZHANG Tiantian1 | 1. SKL of Marine Food Processing & Safety Control, College of Food Science and Engineering, Ocean
University of China, Qingdao 266404,Shandong, China
2.Sanya Institute of Oceanography,
Ocean University of China, Sanya 572024, Hainan, China |
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| Abstract: |
| In order to reveal the phospholipids compositions and differences between male and female Hippocampus abdominalis, the lipids of female and male Hippocampus abdominalis were extracted by solvent extraction method, then phospholipids in the lipids were extracted by cold acetone. Moreover, the different phospholipids fractions were separated by thin-layer chromatography and silica gel column chromatography, then the fatty acid composition of phospholipids and the different phospholipids fractions from female and male Hippocampus abdominalis were analyzed by gas chromatography. The results showed that the female and male Hippocampus abdominalis were rich in phospholipids, accounting for 18.11% and 18.72% of the total lipids, respectively. The phospholipids of Hippocampus abdominalis included phosphatidylethanolamine, phosphatidylcholine, lysophosphatidylcholine and sphingomyelin, among which phosphatidylcholine was the most abundant. The phospholipids of Hippocampus abdominalis identified 20 kinds of fatty acids, and the content of unsaturated fatty acids was higher than that of saturated fatty acids. Furthermore, the total content of marine characteristic n-3 polyunsaturated fatty acids DHA and EPA in female and male Hippocampus abdominalis phospholipids were 18.30% and 19.61%, respectively. The saturated fatty acids of phosphatidylethanolamine, phosphatidylcholine and lysophosphatidylcholine in both male and female Hippocampus abdominalis were all dominated by C16∶ 0 and C18∶ 0, whereas those in sphingomyelin were dominated by C14∶ 0 and C16∶ 0. The monounsaturated fatty acids of the four phospholipid fractions of the male and female Hippocampus abdominalis were all dominated by C18∶ 1, and the polyunsaturated fatty acids were all dominated by EPA and DHA. The total contents of DHA and EPA were more than 16%, except for lysophosphatidylcholine in male Hippocampus abdominalis. In conclusion, phospholipids composition of Hippocampus abdominalis is diverse, with abundant marine characteristic n-3 polyunsaturated fatty acids and high nutritional value. |
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