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| Endophytic fungi producing nervonic acid from Macaranga indica seeds |
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Received:April 18, 2025 Revised:December 22, 2025 Accepted:May 21, 2025
Published：July 20, 2026
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| DOI：10.19902/j.cnki.zgyz.1003-7969.250200 |
| KeyWord:endophyte isolation and identification fatty acid nervonic acid Macaranga indica |
| FundProject:云南省中青年学术和技术带头人后备人才项目（202105AC160083） |
| Author Name | Affiliation | | YAO Yuling1，2, GUO Juan2, QIANG Qi2, PANG Zhiqiang2， LI Shaopeng3, TIAN Bo2 | 1.College of Chinese Medicine, Yunnan University of Traditional Chinese Medicine, Kunming 650500,
China
2.Xishuangbanna Tropical Botanical Garden, Chinese Academy of Sciences, Xishuangbanna
666303,Yunnan,China
3.Pu′er Tea College, West Yunnan University of Applied Sciences,
Pu′er 671006,Yunnan,China |
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| Abstract: |
| In order to explore the potential of the Macaranga indica as a microbial resource for nervonic acid production, different culture media were used to cultivate the seeds and seed oil of Macaranga indica, and the isolated and purified endophytes were identified by 16S rRNA and ITS amplicon sequencing. The fatty acid composition of the purified endophytic fungi was determined, and further erucic acid was added as a substrate to the culture medium to detect their fatty acid composition after cultivation. The results showed that a total of 153 strains of bacteria belonging to 17 genera and 26 species were isolated from the seeds of the Macaranga indica. There were a total of 168 fungi, belonging to 26 genera and 47 species. The fatty acid composition of endophytic fungi was mainly composed of palmitic acid, oleic acid, and linoleic acid, nervonic acid was not detected, but erucic acid (a precursor of nervonic acid) was detected from 6 endophytic fungi. Among them, when erucic acid was added as a substrate for cultivation, the Aspergillus candidus was able to produce 132% of nervonic acid. In summary, endophytic fungi from Macaranga indica seeds may facilitate the accumulation of nervonic acid, indicating the potential of these seeds as a microbial resource for nervonic acid production. |
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