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| Synthesis of campesterol by recombinant Yarrowia lipolytica |
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| DOI： |
| KeyWord:phytosterol campesterol ergosterol Yarrowia lipolytica |
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| Abstract: |
| Campesterol as one of the phytosterols has important physiological activity. In order to construct engineered strain which could synthesize campesterol, the ergosterol synthesis gene ERG5 was disrupted in Yarrowia lipolytica polf, promoting the accumulation of precursor product ergosta-5,7,24-trienol. Then the codon-optimized DHCR7 gene from Xenapus laevis was expressed. Under the coefficient of DHCR7 and endogenous ERG4 in polf,the ergosta-5,7,24-trienol was transformed into campesterol. The fermentation products of the recombinant strain were analyzed by GC-MS. The results showed that engineered strain which could produce campesterol was obtained, and the maximum yield was 0.485 mg/g( based on dry cell weight). Thus, an engineered strain that could biosynthesize campesterol was successfully constructed, which provided a new way for the biosynthesis of phytosterols. |
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