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| Application of IAC in pretreatment for determination of organophosphorus pesticide residues in vegetable oil |
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| DOI：10.12166/j.zgyz.1003-7969/2020.04.027 |
| KeyWord:vegetable oil organophosphorus pesticide residues pretreatment IAC |
| FundProject:浙江省重点研发计划项目(2018C02016)；浙江省科技厅省属科研院所专项（2018F10005） |
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| Abstract: |
| A fast and effective method was established for the cleanup of organophosphorus pesticide residues in vegetable oil .Organophosphorus pesticides in vegetable oil samples were extracted with different organic reagents. After freezing and centrifugation, the supernatant was purified by multi-functional immunoaffinity column (MycoSep 226 AflaZon+). The concentrated liquid was determined by gas chromatography and quantified by external standard method. Three kinds of extraction reagents such as acetone, n-hexane and acetonitrile, and three extraction methods such as homogenization, vortex and ultrasound were screened by the recovery index of 13 organophosphorus pesticides pesticides. The results showed that acetonitrile was the best extraction reagent with the recovery of 81.3%-101.5%. The recoveries of three extraction methods were all above 76.6%, and the recoveries of homogenization and vortex were all above 81%. Among them, vortex was more convenient. The linear relationship of 13 organ ophosphorus pesticides regression equation was good in the range of 0.02-0.50 mg/L, and the correlation coefficient (R2) was not lower than 0.999. The detection limit was 0.005-0.010 mg/kg. The average recoveries of low, medium and high standard additions of 0.04, 0.08 mg/kg and 0.20 mg/kg were 80.3%-1021%, and the RSD was not more than 3.93%. This method had the characteristics of saving reagents, high detection accuracy, low pollution, simple operation of column purification completed within 1 min. The method extablished was suitable for batch detection of organophosphorus pesticide residues in vegetable oil. |
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