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| 亚麻籽油对肥胖小鼠视网膜的保护作用研究 |
| Preventive effect of flaxseed oil against diabetic retinopathy in obese mice |
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| DOI： |
| 中文关键词: 亚麻籽油 糖尿病视网膜病变 肥胖 |
| 英文关键词:flaxseed oil diabetic retinopathy obese |
| 基金项目:陕西省自然科学基础研究计划项目（2017JM8060）；陕西省教育厅专项科研计划项目（19JK0772）；西安医学院2018年配套基金项目（2018PT60）；省级、校级大学生创新创业训练计划项目（S201911840002，2018DC-62） |
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| 中文摘要: |
| 为了研究亚麻籽油对肥胖小鼠视网膜的作用，将小鼠随机分为正常膳食组(标准鼠粮)、高脂膳食组（拌合猪油鼠粮），饲喂8周，分别检测体重及胰岛素抵抗情况，分析其肥胖性糖尿病诱发情况。将诱发成功的小鼠进一步分为两组，即高脂膳食组（拌合猪油鼠粮）及亚麻籽油组（拌合猪油+亚麻籽油鼠粮）。与正常膳食组同步再饲喂8周后，分别检测小鼠的体重、胰岛素抵抗情况，同时处死小鼠，取视网膜组织，以ELISA法检测其视网膜炎症因子IL-10及视网膜血管内皮生长因子VEGF水平。结果表明：连续饲喂8周后，高脂膳食组小鼠出现明显的肥胖、胰岛素抵抗症状，肥胖性糖尿病小鼠造模成功；此后再饲喂8周，相对高脂膳食组，亚麻籽油组小鼠肥胖状况未得到改善(P＞0.05)，但胰岛素抵抗状况明显改善(P＜0.05)，IL-10及VEGF水平明显降低(P＜0.05)。研究结果说明含亚麻籽油的膳食饲喂可以改善高脂膳食引起的肥胖小鼠的视网膜损伤。 |
| 英文摘要: |
| The preventive effect of flaxseed oil against diabetic retinopathy in obese mice was studied. KM mice were randomly divided into normal dietary group (standard mice grain), high-fat diet group (high lard mice grain). After feeding for eight weeks, the weight and insulin resistance were examined respectively to analyze the induced condition of obesity diabetes. The obese mice were further divided into two groups: the high-fat diet group (lard) and the flaxseed oil group (lard+flaxseed oil). After eight weeks of simultaneous feeding with the normal diet group, the weight and insulin resistance of three groups of mice were examined respectively. The mice were executed, the retina tissue was taken, and the levels of retinal inflammatory factor IL-10 and retinal vascular endothelial growth factor VEGF were detected by ELISA. The results showed that after eight weeks of continuous feeding, mice in the high-fat diet group showed significant obesity, insulin resistance symptoms, and the obese diabetic mice model was successfully introduced, and after eight weeks of feeding, compared with high-fat diet group, the obesity status of mice in the flaxseed oil group did not improve (P＞0.05), but the insulin resistance was significantly improved(P＜0.05), the levels of IL-10 and VEGF were significantly reduced (P＜0.05). The results indicated that dietary feeding with flaxseed oil could improve retinal damage in obese mice caused by high-fat diet. |
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