| Abstract：A gas chromatography detection method was established, which could simultaneously quantitatively analyze the tocopherols (α-tocopherol, (β+γ)-tocopherol, δ-tocopherol),phytosterols (rapeseed sterol, campesterol, stigmasterol, β-sitosterol) and squalene in soybean oil deodorized distillate. With cetyl palmitate as the internal standard substance, the sample was directly dissolved in the internal standard solution, and the HP-1 column was used to separate various components. The results showed that the separation of various components was good. The tocopherol, phytosterol and squalene showed good linear relationships in the ranges of 0.4-6.4 mg/mL, 0.5-8.0 mg/mL and 0.4-4.0 mg/mL respectively, the relative coefficients were all greater than or equal to 0.999, and their average recovery rates were 95.38%-102.78%,96.40%- 101.24%,96.14%-104.43% respectively. The relative standard deviations were all lower than 10%, and their detection limits were 0.08, 0.15 mg/L and 0.05 mg/L, respectively. The method had the items of simple pretreatment, good reproducibility, high sensitivity, and accurate and reliable results, which could be applied in the simultaneous determination of tocopherol, phytosterol and squalene in soybean oil deodorized distillate. |
