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| Molecular identification and enzymatic properties of an organic solvent tolerant lipase-producing Burkholderia sp. strain |
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| DOI： |
| KeyWord:strain lipase molecular identification enzymatic activity heat-resistance resistance to organic solvents acid-resistanc |
| FundProject:国家自然科学基金项目（31900122）;家蚕基因组生物学国家重点实验室开放课题（SKLSGB-ORP202106）;安徽省自然科学基金项目（1908085QC124）；安徽省优秀拔尖人才项目（gxgwfx2020060）;大学生创新创业训练项目（201910375013，S202110375034，202210375095） |
| Author Name | Affiliation | | BAI Xiaohui1,2, FAN Yan1,2, ZHOU Juan3, XU Qinghui3, SHAO Yang1,
ZHANG Xin1, HONG Guangming4, ZHENG Zhi5 | 1.College of Life and Environment Science, Huangshan University, Huangshan 245041, Anhui, China
2.School of Life Science and Technology, Henan Institute of Science and Technology, Xinxiang 453003,
Henan, China
3.Huangshan Institute of Product Quality Inspection, Huangshan 245000, Anhui, China
4.Huangshan Jialong Green Food Co. , Ltd. , Huangshan 245421, Anhui, China
5.School of Food and
Biological Engineering, Hefei University of Technology, Hefei 230601, China |
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| Abstract: |
| In order to screen the lipase-producing strain with potential applications, the solid medium containing tributyrin was used to screen the lipase-producing strain from the soil of pine forest in Huangshan. Then the lipase-producing strain were identified by 16S rDNA sequencing and the enzymatic activity parameters and properties of the lipase produced were determined. The results showed that the lipase-producing strain HSU-7 selected was identified as Burkholderia sp.. The optimum reaction temperature and pH for the lipase produced by the strain HSU-7 were 45 ℃ and 5, respectively, revealing that it was an acid-resistant and medium-temperature lipase. The enzymatic activity of lipase produced by strain HSU-7 was the highest at 72.22 U/mL after 5 d of fermentation. Meanwhile, K+, Ca2+, and Mg2+ significantly promoted the enzymatic activity of the lipase produced by strain HSU-7, while Ni2+, Cu2+, Fe3+, and Zn2+ had some inhibition effects on the enzymatic activity. The lipase could tolerate the organic solvents methanol, ethanol, isopropanol and dimethyl sulfoxide, but Triton X-100 significantly inhibited the activity of the lipase. In addition, the lipase could tolerate 65 ℃. In summary, the strain HSU-7 can produce lipase with high yield, and the lipase has strong thermal stability and can tolerate a variety of organic solvents, so the strain has good industrial application value. |
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