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| Optimization and kinetics modeling of the hydrolysis process of Swida wilsoniana oil |
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| DOI： |
| KeyWord:Swida wilsoniana oil lipase fatty acid kinetics modeling |
| FundProject:湖南省创新平台与人才计划（2022PT1004）；湖南省林业科技项目（XLKY202205）；长沙市科技计划——“揭榜挂帅”重大科技项目（kq2301004） |
| Author Name | Affiliation | | KUANG Zengmin1,2, ZHANG Yu1, LI Changzhu2,3,4, XIAO Jingjing2,3,4,
XIAO Zhihong2,3,4,5, LI Peiwang2,3,4, NIE Suli2, CHAO Yan2 | 1.College of Food Science and Technology, Hunan Agricultural University, Changsha 410128, China
2.State Key Laboratory of Utilization of Woody Oil Resources, Hunan Academy of Forestry, Changsha
410004, China
3.Hunan Province Key Laboratory of Oil & Fats Molecular Structure and Function,
Changsha 410004, China
4.Key Laboratory of National Forestry and Grassland Administration on
Utilization Science for Southern Woody Oil Resources, Changsha 410004, China
5.Changsha
Institute of Modern Food Innovation, Hunan Agricultural University, Changsha 410128, China |
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| Abstract: |
| In order to provide reference for enzyme-catalyzed hydrolysis of Swida wilsoniana oil to prepare fatty acids, immobilized lipases Lipozyme RMIM, Lipozyme TLIM, Novozym 435 and free lipase F1 were used as catalysts,the Swida wilsoniana oil was used as substrate, the effects of lipase type, reaction time, reaction temperature, water dosage and enzyme dosage on the hydrolysis rate of Swida wilsoniana oil were investigated. In addition, the hydrolysis kinetics of Swida wilsoniana oil was analyzed. The results showed that the optimal conditions for enzyme-catalyzed hydrolysis of Swida wilsoniana oil were 60% water (based on the mass of water-oil substrate), 5% Lipozyme RMIM (based on the mass of water-oil substrate), reaction temperature 60 ℃ and reaction time 24 h. Under these conditions, the hydrolysis rate of Swida wilsoniana oil could reach 98.08%. The kinetics fitting results showed that the Mi constant (Km) was 0.108 4 mL/g and the maximum reaction rate (νm) was 0.381 4 mg/(g·mL·h) when Lipozyme RMIM was used as catalyst for hydrolysis of Swida wilsoniana oil under optimal conditions. In conclusion, lipase Lipozyme RMIM can efficiently catalyze the hydrolysis of Swida wilsoniana oil. |
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